Concentration: Supplied at ~ 3,000 U/mL
Expression: Purified from Bacillus sp.
Specificity: Hydrolysis of α-1,4 glucosidic linkages in linear α-1,4 glucan (e.g. amylose regions in starch).
Specific Activity: ~ 170 U/mg (40oC, pH 6.5 on Ceralpha reagent)
Unit Definition: One Unit of α-amylase activity is defined as the amount of enzyme required to release one μmole of p-nitrophenol from blocked p-nitrophenyl-maltoheptaoside per minute (in the presence of excess α-glucosidase) at pH 6.5 and 40℃.
Temperature Optima: 100℃
pHOptima: 7