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纤维素酶检测试剂盒(CELLG5 Method)

英文名称:

Cellulase Assay Kit (CELLG5 Method)

商品编号:

CFHN-K-CELLG5-4V

规格型号:

120/240assays(manual)/480(auto-analyser)

Megazyme

用途:

特性量值:

有效期:

单位:

级别:

标准价:

7238.00

优惠价:

数量:



库存与货期:

 订货

储存条件:

 4℃保存

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(CELLG5 Method)

The CELLG5 assay reagent for the measurement of
endo-cellulase (endo-1,4-β-glucanase) contains two components;
1) 4,6-O-(3-Ketobutylidene)-4-nitrophenyl-β-D-cellopentaoside (BPNPG5) and 2) thermostable β-glucosidase. The ketone blocking group prevents any hydrolytic action by the
β-glucosidase on BPNPG5.  Incubation with an endo-cellulase generates a non-blocked colourimetric oligosaccharide that is rapidly hydrolysed by the ancillary β-glucosidase. The rate of formation of 4-nitrophenol is therefore directly related to the hydrolysis of BPNPG5 by the endo-cellulase. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH 9.0).
The CELLG5 assay represents a huge step forward in the methodology for the measurement of cellulase that traditionally relied on substrates such as CM-cellulose, Avicel, cellooligosaccharides, filter paper or dyed polysaccharides including CMC Congo red or cellulose azure.

商品编号 商品名称 品牌/来源 规格型号

产品信息

名称

纤维素酶检测试剂盒(CELLG5 Method)

货号

CFHN-K-CELLG5-4V

品牌/来源

Megazyme

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